The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The SoftMax Pro Software provides integrated instrument control. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 1321N1, a human astrocytoma cell line. Increased fluorescence performance. 635. 312 gain calibration failed. i3. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. With optimized reagents, option to operate the readers. Find regional support. The dynamic range of the assay is about 3. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The SpectraMax ® i3x. 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. They were allowed to attach and grow overnight in a 37°C, 5% CO 2. User-upgradeable applications The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Explore a wealth of applications with the future-ready SpectraMax i3x. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 316 grating motor driver faulty. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Research Article Streptococcus lutetiensis Induces Autophagy via Oxidative Stress in Bovine Mammary Epithelial Cells Peng Chen ,1 Jingyue Yang,1 Naiwen Wu,1 Bo Han,1 John P. The SoftMax Pro Software provides integrated instrument control. Thetypeof luciferase(Renilla. Materials and methodsWe would like to show you a description here but the site won’t allow us. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). 2. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Introduction. Spectranet customer portalThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. As. TUNE Tunable Wavelength Detection Cartridge. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 08, 0. each new lot of plates, to ensure that the center of each well is read. Examples: fiPress carefully to engage,fl fiDo not press down. 0´ 104 6. To detect physical stability, Pdots were treated with water and incubated with plants. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. Sunnyvale, CA, USA) with excitation at 475 nm. Patented user-exchangeable cartridge design expands the system's detection capability making it. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Spectramax I3x, supplied by Danaher Inc, used in various techniques. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. 4. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. china@moldev. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. Molecular Devices are known for both the reliability and the wide uses of their products. However, no piece of equipment is immune to breaking down. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable. Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. The chamber. SpectraMaxi3Multi. This way you’ll save time on finding the necessary info. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. The CellTiter-Glo assay from Promega uses luciferase. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Unlock your i3/i3x instrument after shipment. The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. Read height should be optimized for . ) deep, preferably with access on all sides. Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). Find regional support. ATP (“flash” luminescence using . 1. no. , 2014; Xiong et al. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. The assay window spanned four orders of magnitude. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. Promega. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. These robust readers have been placed in labs from Antarctica to the. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 315 can't find zero order. Bioz Stars score: 98/100, based on 1 PubMed citations. applications, including dual luciferase and ATP assays. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Fluorescent Polarization: Solid black plates, as above. 2C). Learn more about its features, specifications, and applications in this user guide. • Removing the transport locks from the PMT shutters and drawers. Anne T. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. See SpectraMax® Microplate Reader User Manual Download Page. 99 to $1999. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 58% for the detection module and 2. The device can be upgraded to include capabilities for Western blotting, imaging and injection. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. SoftMax Pro GxP Compliance Software. This instrument also provides the option of modular upgrades for Western Blotting,. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Pick the best speed, sensitivity, and price for your needs. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Note: Additional readers with similar performance are listed. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. 58% for the detection module and 2. 4 μM), prepared as described in section 2. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). 313 reference gain check fail. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. ZERO BIAS - scores, article reviews, protocol conditions and more. See Unpacking the SpectraMax Paradigm Instrument on page 1. 2. 19 Switch on the SpectraMax i3x plate reader and launch SoftMax Pro software. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. QBT Fatty Acid Transporter Assay. Lower limits of detection for ATP determined using the ATPlite . 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. 22 μ m fi ltration unit (Sigma, cat. The SpectraMax i3 also allows the user to identify antibodies during Elisa Assays. Simplified workflow: Add cellular imaging assays in a small footprint. 2. iD5. In addition, Image J was used to quantify the. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. 200-800 nm using Spectra Max i3X microplate reader. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. . **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. 1. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Capture Flash Assays with Ease. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 1step assay with SpectraMax microplate readers. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. eu@moldev. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Use the 3. ) wide and 65 cm (25. *On the SpectraMax i3x reader only. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. 価格、見積もり、お近くの販売店を. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. It allows you to explore cellular pathways and protein activation and expression in one system. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. SpectraMax Microplate Readers. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves. Hands-on fun and learning that empowers exploring, inventing and sharing. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. ATP (“flash” luminescence using . flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. SpectraMax® iD3/iD5. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Bioz Stars score: 98/100, based on 1 PubMed citations. Reporter Assay. 800. SpectraMax i3, i3x, and iD5 readers. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. SeeSpectraMax MiniMaxImagingCytometeronpage85. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Published Dec 16, 2020 | Updated Dec 16, 2020. 1. 蛍光 発光 ELISA. china@moldev. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. We would like to show you a description here but the site won’t allow us. i3. Eliminate time-dependent substrate addition steps. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. o For example: Ex: 485/Em 525/Cutoff 515 4. Also refer to this Multimode Reader Comparison Chart. The SpectraMax ® i3x reader provides the. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Description 1. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. SpectraMax M5. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. Explore a wealth of applications with the future-ready SpectraMax i3x. The turbidity. Budget-friendly multi-mode microplate reader . Assay setup for a 384-well low-volume plate. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax i3x reader provides the. Multimode Reader Comparison Chart. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Bioz Stars score: 98/100, based on 1 PubMed citations. The corrected absorbance is within 5% of the. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. ISM Equipment Classification (Group 1, Class A) This equipment is. Multimode Readers Comparison Chart. Multimode Readers Comparison Chart. MTT was purchased from Sigma-Aldrich (St. 4 / 5. Download PDF. Check software compatibility with Windows 10 and locate your software installer. Winnersh Triangle. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 4 mg mL −1; 1. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Published Dec 16, 2020 | Updated Dec 16, 2020. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. 001 0. Contact our support team with your questions regarding Molecular Devices products and applications. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. edu) to be added as a trained user in iLab. The treatments were. Quickly and easily expand the capabilities of your SpectraMax Paradigm or i3x Multi-Mode Microplate reader to include Tunable Wavelength detection using the. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 吸収度マイクロプレートリーダー SpectraMax® Mini. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Examples: “Press carefully to engage,” “ Do not. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 6% for the on-board optics (monochromators). The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. 0 mm hex key. 11. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Examples: fiPress carefully to engage,fl fiDo not press down. Multi-Mode Reader. Promega. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. Nuclear Receptor. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. As. , Fisher Scienti fi c, cat. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SpectraMax i3x Multi-Mode Microplate Reader System. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. D. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. VAL014, right table) assays on the SpectraMax i3x reader are shown below. Next,. Prepare your i3/i3x instrument for shipment. Home > Search Results > Danaher Inc > spectramax i3x. The SpectraMax i2X is used for cell migration, cell. How to properly unlock iD series instrument. 00800 665 32860. *On the SpectraMax i3x reader only. Molecular Devicesの全商品を見る. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Microplate reader assays requiring infrared (IR) or red-shifted. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. The calculated LOQ values were 0. Find regional support. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. 4. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. 2 in. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. 9 x 10-15. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The calculated LOQ values were 0. We would like to show you a description here but the site won’t allow us. . Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. 2. Find regional support. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 6 in. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. 6% for the on-board optics (monochromators). View online or download 1 Manuals for Molecular Devices SpectraMax i3x. 제품. Specifically, we present neurite. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. Both Microplate Optimization and Read Height Adjustment were. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. 2. 2. Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. applications, including dual luciferase and ATP assays. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. Fluorescence Reader. ). Flow cytometry was used to measure ROS concentrations, based on fluorescence intensity (FL-1, 530 nm) of 10,000 cells. . SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. 2 x 10-14. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. Table 1. Time-. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Cet appareil ISM est confomre à la norme NMB-001 du Canada. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Downloads. In SoftMax® Pro, the following Automix settings are available for the SpectraMax i3/i3x Instrument: Intensity : Low, Medium, or High. ) deep, preferably with access on all sides. SpectraMax i3x Multi-Mode Detection Platform. User-upgradeable detection modules allow you to expand the capabilities of your microp. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. 305 reference level saturation. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. Review date: 19 Mar 2018 | SpectraMax i3x Multi-Mode Microplate Reader with MiniMax Imaging Cytometer The SpectraMax ® line of user-friendly microplate readers provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreSimilar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. Spectranet customer portalThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. The SpectraMax i3x reader provides the. Key features. Figure 2-23: Bottle holder To install the bottle holder: 1. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Supplier: Molecular Devices. 1. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). 06, 0. Introduction. Description 1. Table 1. com. The assay can accurately test the toxicology of compounds as shown in Figure 1. 1. Get a front row view of your cells. 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. com. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity.